L-Tryptophan is an esential amino acid which important to amino acid balance in animal body and animal growth. It is important to creating neoratransmitter to produce serotonin to transmits signals between nerve cells and reducing stress. It also needed to develop organs and optimalized immun system in animal body. Process L-Tryptophan is commonly sythesized in the plants and microorganisms Shikimic acid or Anthranilate.
Anthranilate will be condensed with phosphoribosylpyrophosphate (PRPP) and obtained pyrophosphate as a by-product. When the ring of the part of ribose is opened and subjected to reductive decarboxylation, It will resulted indole-3-glycerol phosphate, then transformed into indole.
Final process is,Indole and the amino acid serin will be calayzed and change their formation into Tryptophan. In commercial inndustry, Tryptophan is produced by biosynthetic based on
the fermentation of serine and indole using either wild-type or genetically modified bacteria such as B. amyloliquefaciens , B. subtilis, C. glutamicum or E. coli. These strains was choosen due to carry mutations which prevent the reuptake of aromatic amino acids. Benefit